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KMID : 0364819910290060380
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Korean Journal of Microbiology
1991 Volume.29 No. 6 p.380 ~ p.386
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Purification and Some Properties of Soluble Cytochrome c from Methylobacillus sp. Strain SKI
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Kim, Si Wouk/김시옥
Ro, Young Tae/Kim, Young Min/노영태/김영민
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Abstract
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1
Three types of soluble cytochrome c were purified to homogeneity from MethylobaciAus sp. strain SKI which grows only on methanol. Cytochrome c-I was purified 58.5-fold in seven steps. Cytochrome c-II and c-III were purified 57.3- and 122.1-fold in eight steps, respectively. The molecular weights of the cytochrome c-I was determined to be 12,500, while those of the cytochrome c-II and c-111 were 16,000. The isoelectric points of the c-I, c-11, and c-III were found to be 8.8, 6.6, and 6.6, respectively. The spectrum of reduced cytochrome c-I showed a-, fl- , y-peaks at 551.4, 522.2, and 416.6 nm. The peaks for c-11 were found at 551.0, 521.6, and 416.5 nm, while those for c-III were shown at 551.2, 521.8, and 416.0 nm. The spectra of oxidized cytochrome c-I, c-II, and c-Ill showed y-peak at 411.8, 409.0, and 410.2 nm, respectively. The absorption coefficients of a-and y- peak for c-I in the reduced state were determined as 47 and 197 mM-´cm´´, respectively. The coefficients of a- and ypeak for c-l1 were determined to be 43 and 137 mM-´cm-´, while those for c-111 were 41 and 172 mM-´cm-´, respectively. The c-I and c-III were found to bind carbon monoxide.
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